Tips & Hints - Swan Pipette Calibration Service, LLC

Pipetting Suggestions

The top 10 things to consider when pipetting:

Evaluate the condition of the pipette.
Choose a pipette with the appropriate volume range: Pipettes are most accurate at their upper limit.
Select quality tips. Poor quality tips can affect the volume delivered by ± 1%.
Correctly set the micrometer. Refer to the pipette user manual.
Correctly Pre-rinse tip(s) per volume size (typically 3 times).
Utilize the correct tip immersion, time, angle, & depth per volume.
Maintain consistent speed & pressure while aspirating and dispensing.
Be mindful of hand warming effects.
Be conscious of ergonomics.
Proper storage; return counter to upper limit, store upright at < 20°.

There are numerous variables that need to be taken into consideration when establishing a pipette calibration service cycle.

The following 7 variables effecting calibration and maintenance of pipettes are often overlooked:

Frequency in which the pipettes are being utilized (hourly, daily, weekly, etc.).
Number of end users who are operating/sharing each pipette.
Matter being aspirated & dispensed (corrosive materials will accelerate the breakdown of lubricants & parts).
Tips that are utilized (filtered vs. non-filtered).
Manner in which the pipettes are being stored (upright @ < 20° is recommended).
Tolerance limits in place (the stricter the tolerances, the more frequently service will be needed).
Quality of pipettes being utilized. Not all pipette manufacturers are created equal. As the saying goes, you get what you pay for!